eIF4B (Phospho-Ser422) Antibody detects endogenous levels of eIF4B only when phosphorylated at Ser422.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
EIF4B
Database Links
Gene ID: 3716 Swiss-Prot #: P23588
OMIM #: 147795 Unigene #: Hs.207538
Target Modification
Phospho
Modification Sites
H:S422 M:S422
Application Images
Image 1
Immunofluorescence analysis of NIH/3T3 cells, using eIF4B (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using eIF4B (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from NIH/3T3 cells, using eIF4B (Phospho-Ser422) Antibody. The lane on the right is blocked with the phospho peptide.