The antiserum was produced against synthesized peptide derived from human Mst1/2 around the phosphorylation site of Thr183.
Immunogen Range
149-198
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
Mst1/2 (Phospho-Thr183) Antibody detects endogenous levels of Mst1/2 only when phosphorylated at Thr183.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
STK3
Database Links
Gene ID: 6789 Swiss-Prot #: Q13188/Q13043
OMIM #: 604965 Unigene #: Hs.472838
Target Modification
Phospho
Modification Sites
H:T183 M:T183
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using Mst1/2 (Phospho-Thr183) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with H2O2 100uM 15', using Mst1/2 (Phospho-Thr183) Antibody. The lane on the right is blocked with the phospho peptide.