The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
p130 Cas (Phospho-Tyr165) Antibody
Synonyms
BCA1; BCAR1; Breast cancer anti-estrogen resistance 1 protein; CAS; CRK-associated substrate; CRKAS
Immunogen
The antiserum was produced against synthesized peptide derived from human p130 Cas around the phosphorylation site of Tyr165.
Immunogen Range
131-180
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
p130 Cas (Phospho-Tyr165) Antibody detects endogenous levels of p130 Cas only when phosphorylated at Tyr165.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BCAR1
Database Links
Gene ID: 9564 Swiss-Prot #: P56945
OMIM #: 602941 Unigene #: Hs.479747
Target Modification
Phospho
Modification Sites
H:Y165 M:Y169 R:Y263
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p130 Cas (Phospho-Tyr165) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using p130 Cas (Phospho-Tyr165) Antibody. The lane on the right is blocked with the phospho peptide.