The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
PKC alpha (Phospho-Thr638) Antibody
Synonyms
kinase PKC-alpha; KPCA; PKC III; PKC-A; PKC-alpha; PKC-III; PRKACA; PRKCA; Protein kinase C, alpha type
Immunogen
The antiserum was produced against synthesized peptide derived from human PKC alpha around the phosphorylation site of Thr638.
Immunogen Range
606-655
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
PKC alpha (Phospho-Thr638) Antibody detects endogenous levels of PKC alpha only when phosphorylated at Thr638.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PRKCA
Database Links
Gene ID: 5578 Swiss-Prot #: P17252
OMIM #: 176960 Unigene #: Hs.531704/Hs.708867
Target Modification
Phospho
Modification Sites
H:T638 M:T638 R:T638
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC alpha (Phospho-Thr638) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from NIH/3T3 cells treated with UV 15', using PKC alpha (Phospho-Thr638) Antibody. The lane on the right is blocked with the phospho peptide.