The antiserum was produced against synthesized peptide derived from human PKC delta around the phosphorylation site of Tyr313.
Immunogen Range
279-328
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
PKC delta (Phospho-Tyr313) Antibody detects endogenous levels of PKC delta only when phosphorylated at Tyr313.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PRKCD
Database Links
Gene ID: 5580 Swiss-Prot #: Q05655
OMIM #: 176977 Unigene #: Hs.155342
Target Modification
Phospho
Modification Sites
H:Y313 M:Y311 R:Y311
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC delta (Phospho-Tyr313) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HepG2 cells, COLO205 cells and HUVEC cells, using PKC delta (Phospho-Tyr313) Antibody. The lane on the right is blocked with the phospho peptide.