The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
YAP (Phospho-Ser127) Antibody
Synonyms
65 kDa Yes-associated protein; YAP1; YAP65
Immunogen
The antiserum was produced against synthesized peptide derived from human YAP around the phosphorylation site of Ser127.
Immunogen Range
93-142
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
YAP (Phospho-Ser127) Antibody detects endogenous levels of YAP only when phosphorylated at Ser127.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
YAP1
Database Links
Gene ID: 10413 Swiss-Prot #: P46937
OMIM #: 606608 Unigene #: Hs.503692
Target Modification
Phospho
Modification Sites
H:S127 M:S112 R:S112
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right is blocked with the phospho peptide.