PKC-pan (Phospho-Thr497) Antibody detects endogenous levels of PKC-pan only when phosphorylated at Thr497.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunofluorescence analysis of HepG2 cells, using PKC-pan (Phospho-Thr497) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC-pan (Phospho-Thr497) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from NIH/3T3 cells treated with PMA 250ng/ml 15', using PKC-pan (Phospho-Thr497) Antibody. The lane on the right is blocked with the phospho peptide.