The antiserum was produced against synthesized peptide derived from human PTP1B around the phosphorylation site of Ser50.
Immunogen Range
16-65
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
PTP1B (Phospho-Ser50) Antibody detects endogenous levels of PTP1B only when phosphorylated at Ser50.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PTPN1
Database Links
Gene ID: 5770 Swiss-Prot #: P18031
OMIM #: 176885 Unigene #: Hs.417549
Target Modification
Phospho
Modification Sites
H:S50 M:S50 R:S50
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using PTP1B (Phospho-Ser50) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with UV 30', using PTP1B (Phospho-Ser50) Antibody. The lane on the right is blocked with the phospho peptide.