The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Chk1 (Phospho-Ser301) Antibody
Synonyms
CHEK1; Chk1; kinase Chk1
Immunogen
The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser301.
Immunogen Range
271-320
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000
Specificity
Chk1 (Phospho-Ser301) Antibody detects endogenous levels of Chk1 only when phosphorylated at Ser301.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CHK1
Database Links
Gene ID: 1111 Swiss-Prot #: O14757
OMIM #: 603078 Unigene #: Hs.24529
Target Modification
Phospho
Modification Sites
H:S301 M:S301 R:S301
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser301) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells, using Chk1 (Phospho-Ser301) Antibody. The lane on the right is blocked with the phospho peptide.