The antiserum was produced against synthesized peptide derived from human Coagulation Factor III around the phosphorylation site of Ser290.
Immunogen Range
246-295
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
Coagulation Factor III (Phospho-Ser290) Antibody detects endogenous levels of Coagulation Factor III only when phosphorylated at Ser290.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
F3
Database Links
Gene ID: 2152 Swiss-Prot #: P13726
OMIM #: 134390 Unigene #: Hs.62192
Target Modification
Phospho
Modification Sites
H:S290
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using Coagulation Factor III (Phospho-Ser290) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from RAW264.7 cells treated with TNF 20ng/ml 30', using Coagulation Factor III (Phospho-Ser290) Antibody. The lane on the right is blocked with the phospho peptide.