The antiserum was produced against synthesized peptide derived from human CRYAB around the phosphorylation site of Ser45.
Immunogen Range
21-70
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
CRYAB (Phospho-Ser45) Antibody detects endogenous levels of CRYAB only when phosphorylated at Ser45.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CRYAB
Database Links
Gene ID: 1410 Swiss-Prot #: P02511
OMIM #: 123590/608810 Unigene #: Hs.408767
Target Modification
Phospho
Modification Sites
H:S45 M:S45 R:S45
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human heart, using CRYAB (Phospho-Ser45) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with anisomycin 25ug/ml 30', using CRYAB (Phospho-Ser45) Antibody. The lane on the right is blocked with the phospho peptide.