The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
MCM4 (Phospho-Ser54) Antibody
Synonyms
CDC21 homolog; DNA replication licensing factor MCM4; P1-CDC21
Immunogen
The antiserum was produced against synthesized peptide derived from human MCM4 around the phosphorylation site of Ser54.
Immunogen Range
20-69
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
MCM4 (Phospho-Ser54) Antibody detects endogenous levels of MCM4 only when phosphorylated at Ser54.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MCM4
Database Links
Gene ID: 4173 Swiss-Prot #: P33991
OMIM #: 602638 Unigene #: Hs.460184
Target Modification
Phospho
Modification Sites
H:S54 M:S53
Application Images
Image 1
Western blot analysis of lysates from COS7 cells treated with nocodazole 1ug/ml 16h, using MCM4 (Phospho-Ser54) Antibody. The lane on the right is blocked with the phospho peptide.