The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Estrogen Receptor-alpha (Phospho-Ser305) Antibody
Synonyms
ER, ESR, ESR1, ESTR, ESTRA, Estradiol receptor, Estrogen receptor, NR3A1
Immunogen
The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-alpha around the phosphorylation site of Ser305.
Immunogen Range
276-325
Clonality
Polyclonal
Application Dilutions
IF: 1:100~1:500 ELISA: 1:20000
Specificity
Estrogen Receptor-alpha (Phospho-Ser305) Antibody detects endogenous levels of Estrogen Receptor-alpha only when phosphorylated at Ser305.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ESR1
Database Links
Gene ID: 2099 Swiss-Prot #: P03372
OMIM #: 133430 Unigene #: Hs.208124
Target Modification
Phospho
Modification Sites
H:S305
Application Images
Image 1
Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using Estrogen Receptor-alpha (Phospho-Ser305) Antibody. The picture on the right is blocked with the phospho peptide.