The antiserum was produced against synthesized peptide derived from human FAS around the phosphorylation site of Tyr291.
Immunogen Range
257-306
Clonality
Polyclonal
Application Dilutions
IF: 1:100~1:500 ELISA: 1:1000
Specificity
FAS (Phospho-Tyr291) Antibody detects endogenous levels of FAS only when phosphorylated at Tyr291.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
FAS
Database Links
Gene ID: 355 Swiss-Prot #: P25445
OMIM #: 134637/601859 Unigene #: Hs.244139
Target Modification
Phospho
Modification Sites
H:Y291
Application Images
Image 1
Immunofluorescence analysis of COS7 cells, using FAS (Phospho-Tyr291) Antibody. The picture on the right is blocked with the phospho peptide.