The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Smad3 (Phospho-Thr179) Antibody
Synonyms
JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3; Mothers against decapentaplegic homolog 3; SMAD 3; Smad 3
Immunogen
The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Thr179.
Immunogen Range
145-194
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
Smad3 (Phospho-Thr179) Antibody detects endogenous levels of Smad3 only when phosphorylated at Thr179.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human heart, using Smad3 (Phospho-Thr179) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HeLa cells treated with TNF 20ng/ml 2', using Smad3 (Phospho-Thr179) Antibody. The lane on the right is blocked with the phospho peptide.