The antiserum was produced against synthesized peptide derived from human Tryptophan Hydroxylase around the phosphorylation site of Ser58.
Immunogen Range
26-75
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
Tryptophan Hydroxylase (Phospho-Ser58) Antibody detects endogenous levels of Tryptophan Hydroxylase only when phosphorylated at Ser58.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
TPH1
Database Links
Gene ID: 7166 Swiss-Prot #: P17752
OMIM #: 191060 Unigene #: Hs.591999
Target Modification
Phospho
Modification Sites
H:S58 M:S61 R:S58
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using Tryptophan Hydroxylase (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with paclitaxel 1uM 24h, using Tryptophan Hydroxylase (Phospho-Ser58) Antibody. The lane on the right is blocked with the phospho peptide.