The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Vinculin (Phospho-Tyr821) Antibody
Synonyms
Metavinculin; VCL; VINC
Immunogen
The antiserum was produced against synthesized peptide derived from human Vinculin around the phosphorylation site of Tyr821.
Immunogen Range
786-835
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000
Specificity
Vinculin (Phospho-Tyr821) Antibody detects endogenous levels of Vinculin only when phosphorylated at Tyr821.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
VINC
Database Links
Gene ID: 7414 Swiss-Prot #: P18206
OMIM #: 193065/611407 Unigene #: Hs.643896
Target Modification
Phospho
Modification Sites
H:Y821 M:Y821 R:Y822
Application Images
Image 1
Immunofluorescence analysis of HepG2 cells, using Vinculin (Phospho-Tyr821) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vinculin (Phospho-Tyr821) Antibody. The picture on the right is blocked with the phospho peptide.