GIRK1/KIR3.1/KCNJ3 (Phospho-Ser185) Antibody detects endogenous levels of GIRK1/KIR3.1/KCNJ3 only when phosphorylated at Ser185.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
KCNJ3
Database Links
Gene ID: 3760 Swiss-Prot #: P48549
OMIM #: 601534 Unigene #: Hs.591606
Target Modification
Phospho
Modification Sites
H:S185 M:S185 R:S185
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using GIRK1/KIR3.1/KCNJ3 (Phospho-Ser185) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using GIRK1/KIR3.1/KCNJ3 (Phospho-Ser185) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from RAW264.7 cells treated with Insulin 0.01U/ml 15', using GIRK1/KIR3.1/KCNJ3 (Phospho-Ser185) Antibody. The lane on the right is blocked with the phospho peptide.