The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
GRB10 (Phospho-Tyr67) Antibody
Synonyms
GRB10 adaptor protein; GRBIR; Growth factor receptor-bound protein 10; Insulin receptor binding protein GRB-IR; KIAA0207
Immunogen
The antiserum was produced against synthesized peptide derived from human GRB10 around the phosphorylation site of Tyr67.
Immunogen Range
33-82
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000
Specificity
GRB10 (Phospho-Tyr67) Antibody detects endogenous levels of GRB10 only when phosphorylated at Tyr67.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
GRB10
Database Links
Gene ID: 2887 Swiss-Prot #: Q13322
OMIM #: 601523 Unigene #: Hs.164060
Target Modification
Phospho
Modification Sites
H:Y67
Application Images
Image 1
Immunofluorescence analysis of HepG2 cells, using GRB10 (Phospho-Tyr67) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from NIH/3T3 cells treated with Insulin 0.01U/ml 15', using GRB10 (Phospho-Tyr67) Antibody. The lane on the right is blocked with the phospho peptide.