The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
IkappaB-alpha (Phospho-Tyr305) Antibody
Synonyms
I-kappa-B-alpha; IkappaBalpha; IKBA; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappaB inhibitor alpha; NFKBI; NFKBIA; RL/IF-1
Immunogen
The antiserum was produced against synthesized peptide derived from human IkappaB-alpha around the phosphorylation site of Tyr305.
Immunogen Range
268-317
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
IkappaB-alpha (Phospho-Tyr305) Antibody detects endogenous levels of IkappaB-alpha only when phosphorylated at Tyr305.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NFKBIA
Database Links
Gene ID: 4792 Swiss-Prot #: P25963
OMIM #: 164008/612132 Unigene #: Hs.81328
Target Modification
Phospho
Modification Sites
H:Y305 M:Y302 R:Y302
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human lymph node, using IkappaB-alpha (Phospho-Tyr305) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with nocodazole 1ug/ml 16h, using IkappaB-alpha (Phospho-Tyr305) Antibody. The lane on the right is blocked with the phospho peptide.