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A11005/eBioscience/ALEXA FLUOR 594 GOAT A 0.5 ML
  • A11005/eBioscience/ALEXA FLUOR 594 GOAT A 0.5 ML

A11005/eBioscience/ALEXA FLUOR 594 GOAT A 0.5 ML

價(jià)格: ￥1875.72 市場價(jià): 3126.20
貨號: A11005
品牌: eBioscience
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  • 詳情
  • 使用說明
  • 常見問題

    • MouseIgG(H+L)Cross-AdsorbedSecondaryAntibody(A-11005)inIF

      HumaniPSCStainingHumaniPSCswereculturedonglassslidesunderfeeder-freeconditionsinStemPro?hESCMedium(Product#A1000701).CellswerefixedandpermedwiththeImage-iT?Fixation/PermeABIlizationKit(Product#R37602).Oct4(green)expressionwasvisualizedusinganti-Oct4primaryAbandAlexaFluor?488secondaryAb(Product#A-11034).Tubulin(red)expressionwasvisualizedusinganti-tubulinprimaryAb(Product#32-2600)andAlexaFluor?594secondaryAb(Product#A-11005).Nuclei(blue)werelabeledwithNucBlue?FixedCellStain(Product#R37606).ImageswerecollectedontheFLoid?CellImagingStation(Product#4471136).


      ProductDetails

      TESTEDAPPLICATIONSDILUTION
      FlowCytometry(Flow)1-10μg/mL
      Immunocytochemistry(ICC)1-10μg/mL
      Immunofluorescence(IF)1-10μg/mL
      PUBLISHEDAPPLICATIONS
      Immunohistochemistry(Paraffin)(IHC(P))See6publicationsbelow
      Immunocytochemistry(ICC)See18publicationsbelow
      Immunohistochemistry(Frozen)(IHC(F))See3publicationsbelow
      MiscellaneousPubMed(MISC)See190publicationsbelow
      Immunohistochemistry(IHC)See1publicationsbelow
      SpeciesreactivityMouse
      Host/IsotypeGoat/IgG
      ClassPolyclonal
      TypeSecondaryAntibody
      ImmunogenGammaImmunoglobinsHeavyandLightchains
      ConjugateAlexaFluor?594
      Excitation/EmissionProfileViewspectra 
      FormLiquid
      Concentration2mg/ml
      Purificationpurified
      StoragebufferPBS,pH7.5
      Contains5mMsodiumazide
      Storageconditions4°C,storeindark
      RRIDAB_2534073
      TargetIgG
      CrossAdsorptionAgainsthumanIgGandhumanserumpriortoconjugation
      AntibodyFormWholeAntibody

      ProductSpecificInformation

      Tominimizecross-reactivity,thesegoatanti-mouseIgGwholeantibodieshavebeencross-adsorbedagainsthumanIgGandhumanserumpriortoconjugation.Cross-adsorptionorpre-adsorptionisapurificationsteptoincreasespecificityoftheantibodyresultinginhighersensitivityandlessbackgroundstaining.Thesecondaryantibodysolutionispassedthroughacolumnmatrixcontainingimmobilizedserumproteinsfrompotentiallycross-reactivespecies.Onlythenonspecific-bindingsecondaryantibodiesarecapturedinthecolumn,andthehighlyspecificsecondariesflowthrough.Thebenefitsofthisextrastepareapparentinmultiplexing/multicolor-stainingexperiments(e.g.,flowcytometry)wherethereispotentialcross-reactivitywithotherprimaryantibodiesorintissue/cellfluorescentstainingexperimentswheretherearemaybethepresenceofendogenousimmunoglobulins.

      AlexaFluordyesareamongthemosttrustedfluorescentdyesavailabletoday.Invitrogen?AlexaFluor594dyeisabright,red-fluorescentdyewithexcitationideallysuitedtothe594nmlaserline.Forstablesignalgenerationinimagingandflowcytometry,AlexaFluor594dyeispH-insensitiveoverawidemolarrange.Probeswithhighfluorescencequantumyieldandhighphotostabilityallowdetectionoflow-abundanceBIOLOGicalstructureswithgreatsensitivity.AlexaFluor594dyemoleculescanbeattachedtoproteinsathighmolarratioswithoutsignificantself-quenching,enablingbrighterconjugatesandmoresensitivedetection.Thedegreeoflabelingforeachconjugateistypically2-8fluorophoremoleculesperIgGmolecule;theexactdegreeoflabelingisindicatedonthecertificateofanalysisforeachproductlot.

      Usingconjugatesolutions:Centrifugetheproteinconjugatesolutionbrieflyinamicrocentrifugebeforeuse;addonlythesupernatanttotheexperiment.Thisstepwillhelpeliminateanyproteinaggregatesthatmayhaveformedduringstorage,therebyreducingnonspecificbackgroundstaining.Becausestainingprotocolsvarywithapplication,theappropriatedilutionofantibodyshouldbedeterminedempirically.Forthefluorophore-labeledantibodiesafinalconcentrationof1-10μg/mLshouldbesatisfactoryformostimmunohistochemistryandflowcytometryapplications.

      Background/TargetInformation

      WeofferanextensivelineofInvitrogen?secondaryantibodyconjugateswithwell-characterizedspecificityandlabeledwithawideselectionofpremiumfluorescentdyes,includingInvitrogen?AlexaFluor?fluorescentdyes.Fluorescentsecondaryantibodyconjugatesareusefulinthedetection,sorting,orpurificationofitsspecifiedtargetandidealforfluorescencemicroscopyandconfocallaserscanningmicroscopy,flowcytometry,andfluorescentwesterndetection.ThebreadthoffluorescentMarkersweofferallowsourreagentstobetailoredtoalmostanyfluorescentdetectionsystem.

      Secondaryantibodiesmaybeprovidedinthreeformats:wholeIgG,divalentF(ab')2fragments,andmonovalentFabfragments.Becauseofthehighdegreeofconservationinthestructureofmanyimmunoglobulindomains,mostclass-specificsecondaryantibodiesmustbeaffinity-purifiedandcross-adsorbedtoachieveminimalcross-reactionwithotherimmunoglobulins.

      Oursecondaryantibodyconjugatesaremostcommonlypreparedbyimmunizingthehostanimalwithapooledpopulationofimmunoglobulinsfromthetargetspeciesandcanbefurtherpurifiedandmodified(e.g.,immunoaffinitychromatography,antibodyfragmentation,labelconjugation,etc.)togeneratehighlyspecificreagents.Inthefirstroundofpurification,wholeimmunoglobulinsbindingtotheimmunizingantibodyarerecoveredandmainlyconsistofthe~150-kDaIgGclass.Furtherpurification,forexample,withProteinAorG,removesallunwantedimmunoglobulinclassesexcepttheaffinity-purifiedantibodiesthatreactwiththetarget-specificimmunoglobulinheavyand/orlightchains.

      ForResearchUseOnly.Notforuseindiagnosticprocedures.Notforresalewithoutexpressauthorization.
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