The antiserum was produced against synthesized peptide derived from human PIP5K around the phosphorylation site of Ser307.
Immunogen Range
273-322
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
Specificity
PIP5K (Phospho-Ser307) Antibody detects endogenous levels of PIP5K only when phosphorylated at Ser307.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PIKFYVE
Database Links
Gene ID: 200576 Swiss-Prot #: Q9Y2I7
OMIM #: 121850/609414 Unigene #: Hs.173939
Target Modification
Phospho
Modification Sites
H:S307 M:S318 R:S357
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using PIP5K (Phospho-Ser307) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human brain, using PIP5K (Phospho-Ser307) Antibody. The picture on the right is blocked with the phospho peptide.