The antiserum was produced against synthesized peptide derived from human Casein Kinase I alpha around the phosphorylation site of Tyr321.
Immunogen Range
287-336
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
Casein Kinase I alpha (Phospho-Tyr321) Antibody detects endogenous levels of Casein Kinase I alpha only when phosphorylated at Tyr321.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CSNK1A1
Database Links
Gene ID: 1452 Swiss-Prot #: P48729
OMIM #: 600505 Unigene #: Hs.529862/Hs.712555
Target Modification
Phospho
Modification Sites
H:T321 M:T321 R:T321
Application Images
Image 1
Western blot analysis of lysates from HUVEC cells, using Casein Kinase I alpha (Phospho-Tyr321) Antibody. The lane on the right is blocked with the phospho peptide.