The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Nibrin (Phospho-Ser278) Antibody
Synonyms
Cell cycle regulatory protein P95; NBN; NBS; NIBRIN; NIJMEGEN BREAKAGE syndrome protein 1; p95-NBS1
Immunogen
The antiserum was produced against synthesized peptide derived from human Nibrin around the phosphorylation site of Ser278.
Immunogen Range
251-300
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Specificity
Nibrin (Phospho-Ser278) Antibody detects endogenous levels of Nibrin only when phosphorylated at Ser278.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunofluorescence analysis of NIH/3T3 cells, using Nibrin (Phospho-Ser278) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HUVEC cells treated with Forskolin 40nM 30', using Nibrin (Phospho-Ser278) Antibody. The lane on the right is blocked with the phospho peptide.