The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha/gamma around the phosphorylation site of Tyr467/199.
Immunogen Range
436-485
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000
Specificity
PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody detects endogenous levels of PI3-kinase p85-alpha/gamma only when phosphorylated at Tyr467/199.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunofluorescence analysis of NIH/3T3 cells, using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30', using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The lane on the right is blocked with the phospho peptide.