The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha around the phosphorylation site of Tyr607.
Immunogen Range
573-622
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
PI3-kinase p85-alpha (Phospho-Tyr607) Antibody detects endogenous levels of PI3-kinase p85-alpha only when phosphorylated at Tyr607.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PIK3R1
Database Links
Gene ID: 5295 Swiss-Prot #: P27986
OMIM #: 171833 Unigene #: Hs.132225/Hs.604502
Target Modification
Phospho
Modification Sites
H:Y607 M:Y607 R:Y607
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from rat kidney, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The lane on the right is blocked with the phospho peptide.