Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | ATF2(Phospho-Thr69or51)Antibody |
Synonyms | Activating2;ATF2;cAMPresponseelementbindingproteinCRE-BP1;CREB2;CREBP1;Cyclic-AMP-dependentATF-2;HB16;MXBPprotein |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanATF2aroundthephosphorylationsiteofThr69or51. |
ImmunogenRange | 36-85 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:20000 |
Specificity | ATF2(Phospho-Thr69or51)AntibodydetectsendogenouslevelsofATF2onlywhenphosphorylatedatThr69or51. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | ATF2 |
DatabaseLinks | GeneID: 1386 Swiss-Prot#: P15336 OMIM#: 123811 Unigene#: Hs.592510 |
TargetModification | Phospho |
ModificationSites | H:S69M:S51R:S51 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanbreastcarcinoma,usingATF2(Phospho-Thr69or51)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromLOVOcells,usingATF2(Phospho-Thr69or51)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |