The antiserum was produced against synthesized peptide derived from human Catenin-beta around the phosphorylation site of Thr41/Ser45.
Immunogen Range
Nov-60
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
Catenin-beta (Phospho-Thr41/Ser45) Antibody detects endogenous levels of Catenin-beta only when phosphorylated at Thr41/Ser45.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CTNNB1
Database Links
Gene ID: 1499 Swiss-Prot #: P35222
OMIM #: 116806/132600/155255 Unigene #: Hs.476018
Target Modification
Phospho
Modification Sites
H:T41+S45 M:T41+S45 R:T41+S45
Application Images
Image 1
Western blot analysis of lysates from HepG2 cells, using Catenin-beta (Phospho-Thr41/Ser45) Antibody. The lane on the right is blocked with the phospho peptide.