The antiserum was produced against synthesized peptide derived from human BCL-XL around the phosphorylation site of Ser62.
Immunogen Range
28-77
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
BCL-XL (Phospho-Ser62) Antibody detects endogenous levels of BCL-XL only when phosphorylated at Ser62.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BCL2L1
Database Links
Gene ID: 598 Swiss-Prot #: Q07817
OMIM #: 600039 Unigene #: Hs.516966
Target Modification
Phospho
Modification Sites
H:S62 M:S62 R:S62
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BCL-XL (Phospho-Ser62) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with UV and MDA-MB-435 cells treated with UV, using BCL-XL (Phospho-Ser62) Antibody.