The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1092.
Immunogen Range
1061-1110
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
EGFR (Phospho-Tyr1092) Antibody detects endogenous levels of EGFR only when phosphorylated at Tyr1092.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
EGFR
Database Links
Gene ID: 1956 Swiss-Prot #: P00533
OMIM #: 131550/211980 Unigene #: Hs.488293
Target Modification
Phospho
Modification Sites
H:Y1092 M:Y1092 R:Y1091
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR (Phospho-Tyr1092) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HUVEC cells treated with EGF, using EGFR (Phospho-Tyr1092) Antibody. The lane on the right is blocked with the phospho peptide.