The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1172.
Immunogen Range
1139-1188
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
EGFR (Phospho-Tyr1172) Antibody detects endogenous levels of EGFR only when phosphorylated at Tyr1172.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
EGFR
Database Links
Gene ID: 1956 Swiss-Prot #: P00533
OMIM #: 131550/211980 Unigene #: Hs.488293
Target Modification
Phospho
Modification Sites
H:Y1172 M:Y1172 R:Y1171
Application Images
Image 1
Western blot analysis of lysates from A431 cells treated with EGF 40 muM 10', using EGFR (Phospho-Tyr1172) Antibody. The lane on the right is blocked with the phospho peptide.