The antiserum was produced against synthesized peptide derived from human eIF2 alpha around the phosphorylation site of Ser51.
Immunogen Range
21-70
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
eIF2 alpha (Phospho-Ser51) Antibody detects endogenous levels of eIF2 alpha only when phosphorylated at Ser51.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
EIF2S1
Database Links
Gene ID: 1965 Swiss-Prot #: P05198
OMIM #: 603907 Unigene #: Hs.151777
Target Modification
Phospho
Modification Sites
H:S52 M:S52 R:S52
Application Images
Image 1
Western blot analysis of lysates from K562 cells treated with IFN-alpha 1000U/ml 18h, using eIF2 alpha (Phospho-Ser51) Antibody. The lane on the right is blocked with the phospho peptide.