The antiserum was produced against synthesized peptide derived from human eIF4E around the phosphorylation site of Ser209.
Immunogen Range
168-217
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
eIF4E (Phospho-Ser209) Antibody detects endogenous levels of eIF4E only when phosphorylated at Ser209.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
EIF4E
Database Links
Gene ID: 1977 Swiss-Prot #: P06730
OMIM #: 133440 Unigene #: Hs.249718
Target Modification
Phospho
Modification Sites
H:S209 M:S209 R:S209
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eIF4E (Phospho-Ser209) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with Anisomycin 25ug/ml 30', using eIF4E (Phospho-Ser209) Antibody. The lane on the right is blocked with the phospho peptide.