The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-alpha around the phosphorylation site of Ser106.
Immunogen Range
71-120
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
Estrogen Receptor-alpha (Phospho-Ser106) Antibody detects endogenous levels of Estrogen Receptor-alpha only when phosphorylated at Ser106.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ESR1
Database Links
Gene ID: 2099 Swiss-Prot #: P03372
OMIM #: 133430 Unigene #: Hs.208124
Target Modification
Phospho
Modification Sites
H:S106 M:S110 R:S111
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Estrogen Receptor-alpha (Phospho-Ser106) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from MCF7 cells, using Estrogen Receptor-alpha (Phospho-Ser106) Antibody. The lane on the left is blocked with the phospho peptide.