The antiserum was produced against synthesized peptide derived from human HDAC2 around the phosphorylation site of Ser394.
Immunogen Range
360-409
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
HDAC2 (Phospho-Ser394) Antibody detects endogenous levels of HDAC2 only when phosphorylated at Ser394.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
HDAC2
Database Links
Gene ID: 3066 Swiss-Prot #: Q92769
OMIM #: 605164 Unigene #: Hs.3352
Target Modification
Phospho
Modification Sites
H:S394 M:S394 R:S452
Application Images
Image 1
Western blot analysis of lysates from LOVO cells, using HDAC2 (Phospho-Ser394) Antibody. The lane on the right is blocked with the phospho peptide.