IRS-1 (Phospho-Ser307) Antibody detects endogenous levels of IRS-1 only when phosphorylated at Ser307.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
IRS1
Database Links
Gene ID: 3667 Swiss-Prot #: P35568
OMIM #: 125853/147545 Unigene #: Hs.471508
Target Modification
Phospho
Modification Sites
H:S307 M:S302 R:S302
Application Images
Image 1
Immunofluorescence analysis of COS7 cells, using IRS-1 (Phospho-Ser307) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IRS-1 (Phospho-Ser307) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from K562 cells, using IRS-1 (Phospho-Ser307) Antibody. The lane on the right is blocked with the phospho peptide.