IkappaB-alpha (Phospho-Ser32/Ser36) Antibody detects endogenous levels of IkappaB-alpha only when phosphorylated at Ser32/Ser36.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NFKBIA
Database Links
Gene ID: 4792 Swiss-Prot #: P25963
OMIM #: 164008/612132 Unigene #: Hs.81328
Target Modification
Phospho
Modification Sites
H:S32+S36 M:S32+S36 R:S32+S36
Application Images
Image 1
Immunofluorescence analysis of LOVO cells, using IkappaB-alpha (Phospho-Ser32/Ser36) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-alpha (Phospho-Ser32/Ser36) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from COS7 cells, using IkappaB-alpha (Phospho-Ser32/Ser36) Antibody. The lane on the right is blocked with the phospho peptide.