The antiserum was produced against synthesized peptide derived from human MEK1 around the phosphorylation site of Thr291.
Immunogen Range
261-310
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
MEK1 (Phospho-Thr291) Antibody detects endogenous levels of MEK1 only when phosphorylated at Thr291.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAP2K1
Database Links
Gene ID: 5604 Swiss-Prot #: Q02750
OMIM #: 115150/176872 Unigene #: Hs.145442
Target Modification
Phospho
Modification Sites
H:T292 M:T292 R:T292
Application Images
Image 1
Western blot analysis of lysates from K562 cells, using MEK1 (Phospho-Thr291) Antibody. The lane on the right is blocked with the phospho peptide.