The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Thr421.
Immunogen Range
411-460
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
p70 S6 Kinase (Phospho-Thr421) Antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at Thr421.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RPS6KB1
Database Links
Gene ID: 6198 Swiss-Prot #: P23443
OMIM #: 608938 Unigene #: Hs.463642
Target Modification
Phospho
Modification Sites
H:T444 M:T444 R:T444
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (Phospho-Thr421) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using p70 S6 Kinase (Phospho-Thr421) Antibody. The lane on the left is blocked with the phospho peptide.