The antiserum was produced against synthesized peptide derived from human p53 around the phosphorylation site of Ser366.
Immunogen Range
331-380
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
p53 (Phospho-Ser366) Antibody detects endogenous levels of p53 only when phosphorylated at Ser366.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p53 (Phospho-Ser366) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HeLa cells treated with Adriamycin 0.5ug/ml 24h, using p53 (Phospho-Ser366) Antibody. The lane on the right is blocked with the phospho peptide.