The antiserum was produced against synthesized peptide derived from human SHP-1 around the phosphorylation site of Tyr564.
Immunogen Range
530-579
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:5000
Specificity
SHP-1 (Phospho-Tyr564) Antibody detects endogenous levels of SHP-1 only when phosphorylated at Tyr564.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PTPN6
Database Links
Gene ID: 5777 Swiss-Prot #: P29350
OMIM #: 176883 Unigene #: Hs.63489
Target Modification
Phospho
Modification Sites
H:Y564
Application Images
Image 1
Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using SHP-1 (Phospho-Tyr564) Antibody. The lane on the right is blocked with the phospho peptide.