The antiserum was produced against synthesized peptide derived from human AXL around the phosphorylation site of Tyr691.
Immunogen Range
657-706
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
AXL (Phospho-Tyr691) Antibody detects endogenous levels of AXL only when phosphorylated at Tyr691.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
AXL
Database Links
Gene ID: 558 Swiss-Prot #: P30530
OMIM #: 109135 Unigene #: Hs.590970
Target Modification
Phospho
Modification Sites
H:Y691 M:Y692 R:Y692
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using AXL (Phospho-Tyr691) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 15', using AXL (Phospho-Tyr691) Antibody. The lane on the right is blocked with the phospho peptide.