The antiserum was produced against synthesized peptide derived from human MAP3K7 around the phosphorylation site of Ser439.
Immunogen Range
411-460
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
MAP3K7 (Phospho-Ser439) Antibody detects endogenous levels of MAP3K7 only when phosphorylated at Ser439.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAP3K7
Database Links
Gene ID: 6885 Swiss-Prot #: O43318
OMIM #: 602614 Unigene #: Hs.714773
Target Modification
Phospho
Modification Sites
H:S439 M:S412 R:S439
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using MAP3K7 (Phospho-Ser439) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30', using MAP3K7 (Phospho-Ser439) Antibody. The lane on the right is blocked with the phospho peptide.