The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
MSK1 (Phospho-Ser212) Antibody
Synonyms
Nuclear mitogen- and stress-activated protein kinase 1; 90 kDa ribosomal protein S6 kinase 5; RSK-like protein kinase; RSKL
Immunogen
The antiserum was produced against synthesized peptide derived from human MSK1 around the phosphorylation site of Ser212.
Immunogen Range
181-230
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
MSK1 (Phospho-Ser212) Antibody detects endogenous levels of MSK1 only when phosphorylated at Ser212.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RPS6KA5
Database Links
Gene ID: 9252 Swiss-Prot #: O75582
OMIM #: 603607 Unigene #: Hs.510225
Target Modification
Phospho
Modification Sites
H:S212 M:S211
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MSK1 (Phospho-Ser212) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 5', using MSK1 (Phospho-Ser212) Antibody. The lane on the right is blocked with the phospho peptide.