The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
PKCB (Phospho-Ser661) Antibody
Synonyms
kinase PKC-beta; KPCB; PKC II; PKC-B; PKC-beta; PKC-II; PRKCB; PRKCB1; Protein kinase C, beta type
Immunogen
The antiserum was produced against synthesized peptide derived from human PKCB around the phosphorylation site of Ser661.
Immunogen Range
622-671
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
PKCB (Phospho-Ser661) Antibody detects endogenous levels of PKCB only when phosphorylated at Ser661.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PRKCB
Database Links
Gene ID: 5579 Swiss-Prot #: P05771
OMIM #: 176970 Unigene #: Hs.460355
Target Modification
Phospho
Modification Sites
H:S661 M:S629 R:S661
Application Images
Image 1
Western blot analysis of lysates from HeLa treated with PMA (left), HeLa (middle), and HeLa treated with heat shock (right), using PKCB (Phospho-Ser661) Antibody.