The antiserum was produced against synthesized peptide derived from human Trk C around the phosphorylation site of Tyr516.
Immunogen Range
482-531
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
Trk C (Phospho-Tyr516) Antibody detects endogenous levels of Trk C only when phosphorylated at Tyr516.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NTRK3
Database Links
Gene ID: 4916 Swiss-Prot #: Q16288
OMIM #: 191316 Unigene #: Hs.410969/Hs.706364
Target Modification
Phospho
Modification Sites
H:Y516 M:Y516 R:Y516
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using Trk C (Phospho-Tyr516) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HeLa cells treated with serum 20% 15', using Trk C (Phospho-Tyr516) Antibody. The lane on the right is blocked with the phospho peptide.