The antiserum was produced against synthesized peptide derived from human Androgen Receptor around the phosphorylation site of Tyr363.
Immunogen Range
331-380
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
Androgen Receptor (Phospho-Tyr363) Antibody detects endogenous levels of Androgen Receptor only when phosphorylated at Tyr363.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of lysates from Jurkat cells treated with UV 15', using Androgen Receptor (Phospho-Tyr363) Antibody. The lane on the right is blocked with the phospho peptide.