The antiserum was produced against synthesized peptide derived from human FRS2 around the phosphorylation site of Tyr436.
Immunogen Range
402-451
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
FRS2 (Phospho-Tyr436) Antibody detects endogenous levels of FRS2 only when phosphorylated at Tyr436.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
FRS2
Database Links
Gene ID: 10818 Swiss-Prot #: Q8WU20
OMIM #: 607743 Unigene #: Hs.593446
Target Modification
Phospho
Modification Sites
H:Y436 M:Y436
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using FRS2 (Phospho-Tyr436) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells , using FRS2 (Phospho-Tyr436) Antibody. The lane on the right is blocked with the phospho peptide.