The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
GRB2 (Phospho-Ser159) Antibody
Synonyms
GRB2-associated-binding protein 2; Growth factor receptor bound protein 2-associated protein 2; GRB2-associated binder 2; pp100; KIAA0571
Immunogen
The antiserum was produced against synthesized peptide derived from human GRB2 around the phosphorylation site of Ser159.
Immunogen Range
125-174
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
GRB2 (Phospho-Ser159) Antibody detects endogenous levels of GRB2 only when phosphorylated at Ser159.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
GAB2
Database Links
Gene ID: 9846 Swiss-Prot #: Q9UQC2
OMIM #: 606203 Unigene #: Hs.429434
Target Modification
Phospho
Modification Sites
H:S159 M:S160 R:S160
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using GRB2 (Phospho-Ser159) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HT29 cells treated with serum 20% 15', using GRB2 (Phospho-Ser159) Antibody. The lane on the right is blocked with the phospho peptide.