The antiserum was produced against synthesized peptide derived from human Ephrin B1/B2/B3 around the phosphorylation site of Tyr324.
Immunogen Range
290-339
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at Tyr324.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human brain, using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from K562 cells treated with serum 20% 15', using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The lane on the right is blocked with the phospho peptide.